Abbreviation
Recombinant Human IL4 protein (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.864-4.949 ng/mL.
Alternative Names
Interleukin-4; IL-4; B-cell stimulatory factor 1 (BSF-1); Binetrakin;Lymphocyte stimulatory factor 1; Pitrakinra
Species
Homo sapiens (Human)
Expression Region
25-153aa
Target Protein Sequence
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.864-4.949 ng/mL.
Description
Interleukin-4 drives Th2 polarization and B-cell class switching, making precise control over its concentration essential for dissecting immune regulation without confounding inflammatory signals. This mammalian-expressed protein, spanning the full mature sequence (aa 25–153), demonstrates dose-dependent proliferative activity in TF-1 cells with an ED50 of 1.864–4.949 ng/mL, providing a quantitative benchmark for titrating responses in cell-based functional assays including primary T-cell differentiation, PBMC activation, and B-cell proliferation studies. The endotoxin level below 1.0 EU/μg ensures that observed effects in JAK/STAT and NF-κB signaling pathway experiments reflect IL-4 receptor engagement rather than LPS-driven artifacts, a critical distinction when interpreting cytokine-mediated gene expression changes. Purity exceeding 90% supports its use as a coating antigen in ELISA development, a positive control in Western blot validation of anti-IL-4 antibodies, and a reference standard in multiplex cytokine detection platforms.
