Abbreviation
Recombinant Human CXCR4 protein-VLPs (Active)
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CXCR4 at 10 μg/mL can bind Anti-CXCR4 recombinant antibody (CSB-RA006254MA01HU), the EC50 is 101.7-253.6 ng/mL.
Alternative Names
(CXC-R4)(CXCR-4)(FB22)(Fusin)(HM89)(LCR1)(Leukocyte-derived seven transmembrane domain receptor)(LESTR)(Lipopolysaccharide-associated protein 3)(LAP-3)(LPS-associated protein 3)(NPYRL)(Stromal cell-derived factor 1 receptor)(SDF-1 receptor)(CD antigen CD184)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
1-352aa
Target Protein Sequence
MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
PBS, pH 7.4, 6% trehalose
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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CSB-MP006254HU(F1) is detected by Mouse anti-6*His monoclonal antibody.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CXCR4 at 10 μg/ml can bind Anti-CXCR4 recombinant antibody (CSB-RA006254MA01HU), the EC50 is 101.7-253.6 ng/mL.
Description
CXCR4 is a seven-transmembrane GPCR central to cancer metastasis, HIV entry, and CXCL12-mediated signaling, yet producing it in a conformationally relevant format presents a well-known technical challenge. This full-length human CXCR4 (aa 1–352) is presented on virus-like particles derived from mammalian cells, preserving the native lipid-bilayer context and post-translational modifications essential for proper GPCR folding and epitope accessibility. Functional ELISA confirms binding activity against an anti-CXCR4 recombinant antibody with an EC50 of 101.7–253.6 ng/mL, providing validated support for ligand-receptor interaction assays, competitive inhibition studies, blocking antibody screening, and therapeutic antibody epitope mapping. Endotoxin levels below 1.0 EU/μg, combined with the membrane-embedded presentation format, satisfy criteria typical for drug discovery workflows involving small-molecule or biologic inhibitor screening and align with standards expected in SPR- or BLI-based affinity characterization where conformational integrity is critical.
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