Human copeptin ELISA kit

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Target Background

Name: Human copeptin ELISA kit
Brand: CUSABIO
SKU: CSB-E12130h

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Code: CSB-E12130h

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Citation （22）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, urine, cell culture supernates, tissue homogenates

Detect Range: 78 pg/mL-5000 pg/mL

Sensitivity: 19.5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Product Details
Customer Reviews and Q&A
Target Background

Abbreviation

copeptin

Species

Homo sapiens (Human)

Sample Type

serum, urine, cell culture supernates, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	99
	Range %	92-105
1:4	Average %	97
	Range %	90-106
1:8	Average %	96
	Range %	91-102

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.535	2.752	2.644	2.533
2500	2.012	2.179	2.096	1.985
1250	1.598	1.599	1.599	1.488
625	0.847	0.867	0.857	0.746
312	0.478	0.502	0.490	0.379
156	0.336	0.356	0.346	0.235
78	0.237	0.221	0.229	0.118
0	0.109	0.112	0.111

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Human Copeptin ELISA kit is a valuable tool for detecting copeptin levels in human samples. Copeptin, a glycosylated peptide derived from the C-terminal part of the arginine vasopressin precursor, has been implicated in various medical conditions. This ELISA kit is designed for use with serum, urine, cell culture supernates, and tissue homogenates, offering a wide range of sample types for analysis. With a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL, this kit provides precise measurements within a short assay time of 1-5 hours, requiring only 50-100 µl of sample volume. Operating at a detection wavelength of 450 nm, the assay principle is quantitative, utilizing a sandwich measurement technique. The kit has been utilized in over 21 papers, showcasing its reliability and widespread application in Copeptin-related research.

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■ Q&A

Please provide the following information regarding human copeptin ELISA Kit (CSB-E12130h)
Please confirm the concentration of the stock? (5000pg/ml)

Thanks for your inquiry.
The standard in the kit is lyophilized powder. The concentration after reconstitution by sample diluent is 5000pg/ml.Pls let me know if you have any further questions. Thank you.

Copeptin is associated with mortality in patients with traumatic brain injury /,/,2011
Diagnostic Role of C-reactive Protein, Procalcitonin and Lipopolysaccharide-Binding Protein in Discriminating Bacterial-Community Acquired Pneumonia from 2009 H1N1 Influenza A Infection /,/,2011
Change in plasma copeptin level after acute spontaneous basal ganglia hemorrhage /,/,2011
Detection of copeptin in peripheral blood of patients with aneurysmal subarachnoid hemorrhage /,/,2011
Maternal and neonatal copeptin levels at cesarean section and vaginal delivery /,/,2012
Paediatric perspectives in the diagnosis of polyuria‐polydipsia syndrome F Vivarelli,/,2024
Role of copeptin as dual cardiacMarker strategy for patients with chest pain presented to emergency department Chun Tat Lui. et al,/,2015
Role of copeptin in dual-cardiacMarker strategy for patients with chest pain presented to ED. Lui CT.et al,/,2015
The Evaluation of Serum Copeptin Levels and Some Commonly Seen Thrombophilic Mutation Prevalence in Acute Pulmonary Embolism. Ozturk N.et al,/,2016
Diagnostic and prognostic role of resistin and copeptin in acute ischemic stroke Perovic E.et al,/,2017
Association of copeptin, a surrogate marker of arginine vasopressin, with cerebral vasospasm and delayed ischemic neurologic deficit after aneurysmal subarachnoid hemorrhage Sandra J. Fernandez, MD.et al,/,2018
Incidence of hypotension according to the discontinuation order of vasopressors in the management of septic shock: a prospective randomized trial (DOVSS) Kyeongman Jeon.et al,/,2018
Estimation the activity of Copeptin, insulin, and C-peptide from patients with polycystic ovary syndrome Entedhar R. Sarhat.et al,/,2018
The diagnostic and prognostic value of copeptin in cardiovascular disease, current status, and prospective Seyed Mostafa Parizadeh .et al,/,2018
Prognostic value of copeptin in patients with aneurysmal subarachnoid hemorrhage ZhizhiZuo, et al,/,2019
STUDY SOME IMPORTANT BIOMARKERS IN PATIENTS WITH CHRONIC KIDNEY DISEASE L Fort-Aznar,/,2024
Effects of exercise on serum ischemia-modified albumin, brain natriuretic peptide and copeptin levels in boxers and kick boxers H Memmedov,/,2023
Serum Copeptin, NLPR3, and suPAR Levels among Patients with Autosomal-Dominant Polycystic Kidney Disease with and without Impaired Renal Function V Raptis,/,2020
The Klotho-FGF23-CPP axis and cardiovascular outcomes in diabetic patients with chronic limb threatening ischemia: a prospective study F Biscetti,/,2022
High-intensity interval training ameliorates endothelial dysfunction through adropin, nitric oxide, MR-proADM, and copeptin changes in overweight subjects S Abbasian,/,2022
Evaluation of cardiovascular system and determination of cardiometabolic risk using novel biomarkers in childhood obesity M Deniz,/,2022
Urinary hyaluronidase activity is closely related to vasopressinergic system following an oral water load in men: a potential role in blood pressure regulation and early stages of hypertension development A Calvi,/,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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