Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Product Details

Home
Product
Kit
ELISA Kits
Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Name: Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Brand: CUSABIO
SKU: CSB-E08491r

Promotion

Code: CSB-E08491r

Rate （N/A）
Review （0）
Citation （12）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.312 mIU/mL-20 mIU/mL

Sensitivity: 0.078 mIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Customer Reviews and Q&A
Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Abbreviation

TRACP-5b

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.312 mIU/mL-20 mIU/mL

Sensitivity

0.078 mIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	100
	Range %	97-103
1:4	Average %	93
	Range %	88-97
1:8	Average %	98
	Range %	93-102

Recovery

The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	95	91-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
20	2.076	1.988	2.032	1.887
10	1.292	1.196	1.244	1.099
5	0.804	0.820	0.812	0.667
2.5	0.511	0.521	0.516	0.371
1.25	0.324	0.335	0.330	0.185
0.625	0.282	0.278	0.280	0.135
0.312	0.223	0.212	0.218	0.073
0	0.146	0.144	0.145

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Tartrate-resistant acid phosphatase 5b (TRACP-5b) is a lysosomal enzyme that plays an important role in bone metabolism and remodeling. Osteoclasts primarily secrete this enzyme during bone resorption. It serves as a specific biomarker for osteoclast activity. TRACP-5b levels in circulation reflect the rate of bone resorption, making it a valuable indicator for studying bone turnover dynamics, osteoporosis research, and metabolic bone diseases. Its resistance to tartrate inhibition distinguishes it from other acid phosphatase isoforms, providing specificity for bone-related research applications.

The Rat tartrate-resistant acid phosphatase 5b ELISA Kit (CSB-E08491r) is designed for quantitative measurement of TRACP-5b in rat samples. This sandwich-based assay accommodates serum, plasma, and tissue homogenates with a detection range of 0.312-20 mIU/mL and sensitivity of 0.078 mIU/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, targeting Rattus norvegicus TRACP-5b for research applications.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in bone metabolism research to quantify tartrate-resistant acid phosphatase 5b as a bone resorption marker in rat serum samples. Researchers have applied the assay alongside other bone biomarkers to comprehensively evaluate skeletal metabolism in experimental studies.

• Bone resorption measurement: Used to measure TRACP-5b levels in serum as an indicator of osteoclast activity and bone breakdown processes
• Bone metabolism profiling: Applied in conjunction with bone formation markers like osteocalcin to evaluate overall skeletal turnover
• Biochemical analysis: Incorporated into comprehensive serum biochemical panels for bone-related research studies in rat models