Rat dopamine,DA ELISA Kit

Product Details

Customer Reviews and Q&A

Name: Rat dopamine,DA ELISA Kit
Brand: CUSABIO
SKU: CSB-E08660r

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Code: CSB-E08660r

Rate （4.8）
Review （6）
Citation （53）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Product Details
Customer Reviews and Q&A
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Alternative Names

N/A

Abbreviation

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	86
	Range %	84-96
1:10	Average %	95
	Range %	91-100
1:20	Average %	84
	Range %	81-88
1:40	Average %	92
	Range %	88-96

Recovery

The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	96	94-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.174	2.280	2.227	2.076
5	1.784	1.789	1.787	1.636
2.5	1.250	1.276	1.263	1.112
1.25	0.841	0.875	0.858	0.707
0.625	0.435	0.465	0.450	0.299
0.312	0.287	0.308	0.298	0.147
0.156	0.208	0.223	0.216	0.065
0	0.152	0.150	0.151

Troubleshooting and FAQs

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ELISA Data Analysis

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Rat dopamine,DA ELISA Kit

Description

This Rat dopamine (DA) ELISA Kit was designed for the quantitative measurement of Rat dopamine (DA) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

■ Customer Reviews

Average Rating:

4.8 - 6 reviews

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Sample species : a:1:{i:0;a:2:{i:0;s:3:"Rat";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Review: The DA content was evaluated using the corresponding ELISA kit. A similar pattern was observed in the DA striatal content as presented in the panel I, where 6-OHDA leveled it off, while the co-administration of 6-OHDA with A-779 depleted it markedly.

By Anonymous