Rat Tumor necrosis factor β,TNF-β ELISA KIT

CSB-E07379r

Rate （N/A）
Review （0）
Citation （14）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 7.8 pg/mL-500 pg/mL

Sensitivity: 1.95 pg/mL

Send an Inquiry

Start an on-line Chat

Orders can only be placed from within the U.S.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit

Abbreviation

TNF-β

Uniprot NO.

Q06332

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	96-105
1:2	Average %	102
	Range %	97-107
1:4	Average %	87
	Range %	83-94
1:8	Average %	96
	Range %	91-102

Recovery

The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99108
EDTA plasma (n=4)	88	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.632	2.765	2.699	2.614
250	2.212	2.302	2.257	2.172
125	1.545	1.577	1.561	1.476
62.5	0.765	0.771	0.768	0.683
31.2	0.411	0.423	0.417	0.332
15.6	0.257	0.264	0.261	0.176
7.8	0.176	0.185	0.181	0.096
0	0.084	0.085	0.085

Troubleshooting and FAQs

ELISA kit FAQs

Datasheet & COA

Please contact us to get it.

Description

This Rat TNF-β ELISA Kit was designed for the quantitative measurement of Rat TNF-β protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Citations

Target Background

Function

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

Gene References into Functions

Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID:20092780
TNFbeta-so far only known to be produced by lymphocytes-was demonstrated in alveolar macrophages, bronchial epithelial cells, vascular smooth muscle cells and endothelial cells at the protein and the message level PMID:12849708

Hide All

Subcellular Location

Secreted. Membrane.

Protein Families

Tumor necrosis factor family

Database Links

UNIGENE: Rn.160577

KEGG: rno:25008

STRING: 10116.ENSRNOP00000001111

Promotions