Rat TNF-α ELISA kit

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Name: Rat TNF-α ELISA kit
Brand: CUSABIO
SKU: CSB-E11987r-IS

CSB-E11987r-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid

Detect Range: 15.6 pg/mL-1000 pg/mL

Sensitivity: 3.9 pg/mL

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Alternative Names

Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit

Abbreviation

TNF

Uniprot NO.

P16599

Species

Rattus norvegicus (Rat)

Sample Type

tissue homogenates, cell culture supernates, bronchoalveolar lavage fluid

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	cell culture supernates(n=4)
1:1	Average %	107
	Range %	99-113
1:2	Average %	99
	Range %	96-105
1:4	Average %	103
	Range %	97-109
1:8	Average %	99
	Range %	94-102

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.717

2.634

2.675

2.487

500

1.476

1.467

1.471

1.283

250

0.853

0.908

0.881

0.693

125

0.608

0.629

0.619

0.431

62.5

0.395

0.381

0.388

0.200

31.2

0.281

0.293

0.287

0.099

15.6

0.218

0.207

0.213

0.025

0.191

0.185

0.188

Troubleshooting and FAQs

ELISA kit FAQs

Datasheet & COA

Please contact us to get it.

■ Customer Reviews

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Sodium Phenylbutyrate Ameliorates Ovariectomy-Induced Bone Loss in Rats B Akbaş, G Dinç, A Akbaş, NA Hacım, G Ercan,/,2025
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.

Protein Families

Tumor necrosis factor family

Database Links

UNIGENE: Rn.2275

KEGG: rno:103694380

STRING: 10116.ENSRNOP00000001110

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