Rat Rheumatoid Factor(RF) ELISA Kit

Product Details

Name: Rat Rheumatoid Factor(RF) ELISA Kit
Brand: CUSABIO
SKU: CSB-E13666r

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Code: CSB-E13666r

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma

Detect Range: 15.6 mIU/mL-1000 mIU/mL

Sensitivity: 3.9 mIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Alternative Names

N/A

Abbreviation

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma

Detection Range

15.6 mIU/mL-1000 mIU/mL

Sensitivity

3.9 mIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	104
	Range %	96-108
1:10	Average %	94
	Range %	91-98
1:20	Average %	102
	Range %	98-106
1:40	Average %	88
	Range %	82-96

Recovery

The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	99	92-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
1000	2.646	2.545	2.596	2.443
500	1.975	2.076	2.026	1.873
250	1.284	1.308	1.296	1.143
125	0.751	0.762	0.757	0.604
62.5	0.512	0.501	0.507	0.354
31.2	0.353	0.342	0.348	0.195
15.6	0.285	0.281	0.283	0.130
0	0.153	0.152	0.153

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rat Rheumatoid Factor (RF) ELISA Kit was designed for the quantitative measurement of Rat Rheumatoid Factor (RF) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 mIU/mL-1000 mIU/mL and the sensitivity is 3.9 mIU/mL .