Rat Prostaglandin E2,PG-E2 ELISA Kit

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Code: CSB-E07967r
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates, cell lysates
Detect Range: 0.5 pg/mL-100 pg/mL
Sensitivity: 0.25 pg/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Abbreviation
PG-E2
Species
Rattus norvegicus (Rat)
Sample Type
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.5 pg/mL-100 pg/mL
Sensitivity
0.25 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Competitive
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-99  
1:2 Average % 92  
Range % 87-96  
1:4 Average % 93  
Range % 89-98  
1:8 Average % 105  
Range % 99-109  
Recovery
The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-99  
EDTA plasma (n=4) 101 96-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
100 0.228 0.224 0.226    
25 0.365 0.348 0.357    
8 0.560 0.539 0.550    
2 0.813 0.787 0.800    
0.5 1.258 1.234 1.246    
0 2.031 2.176 2.104    
Troubleshooting and FAQs
Images
  • Rat Prostaglandin E2,PG-E2 ELISA Kit
Description

This Rat PG-E2 ELISA Kit was designed for the quantitative measurement of Rat PG-E2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Competitive ELISA kit, its detection range is 0.5 pg/mL-100 pg/mL and the sensitivity is 0.25 pg/mL.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

Submit a Review here

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:17:"Tissue homogenate";i:1;s:5:"brain";}}

Review: One model group differed from the control group, the other two did not. The operation steps of the product are still very simple, and the standards are also prepared with different concentrations.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:3:"Rat";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: For the detection of pg-e2 in serum, the kit is easy to operate. Six standard samples have been prepared, and the error of self-mixing liquid is small. From the experimental results, it can be seen that the effect is good

By Anonymous

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