Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

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Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

Name: Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit
Brand: CUSABIO
SKU: CSB-EL021339RA

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Code: CSB-EL021339RA

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Citation （4）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell lysates

Detect Range: 34.3 pg/mL-2200 pg/mL

Sensitivity: 8.5 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1

Abbreviation

SIRT1

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates, cell lysates

Detection Range

34.3 pg/mL-2200 pg/mL

Sensitivity

8.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-94
1:2	Average %	102
	Range %	97-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	98
	Range %	92-102

Recovery

The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	99	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2200	2.357	2.272	2.315	2.209
1100	1.976	1.873	1.925	1.819
550	1.582	1.486	1.534	1.428
275	1.141	1.158	1.150	1.044
137.5	0.761	0.757	0.759	0.653
68.75	0.436	0.425	0.431	0.325
34.3	0.262	0.266	0.264	0.158
0	0.107	0.105	0.106

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit is the ideal tool for researchers working in the field of epigenetics and nuclear signaling. This quantitative sandwich assay is designed to detect and quantify the protein expression levels of NAD-dependent protein deacetylase sirtuin-1, also known as SIRT1, in serum, plasma, tissue homogenates, and cell lysates from Rattus norvegicus (Rat).

With a detection range of 34.3 pg/mL-2200 pg/mL and a sensitivity of 8.5 pg/mL, this ELISA kit provides accurate and precise results for your research needs. The assay time is between 1-5 hours, and the required sample volume is only 50-100ul, making it a convenient and efficient tool for your laboratory.

The detection wavelength of 450 nm ensures reliable and consistent results, and the kit comes with all the necessary components and reagents to perform the assay. With more than 3 citations, this kit has been validated and tested by other researchers, providing you with confidence in your results.

■ Customer Reviews

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Potential renoprotective effects of piceatannol in ameliorating the early-stage nephropathy associated with obesity in obese Zucker rats. Llarena M.et al,/,2015
Specific components of child gestures at 14 months are associated with preschoolers' language skills Y Ibara,/,2020
Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells Sedat Kacar,/,2021
Various endurance training intensities improve GFR and Up-regulate AQP2/GSK3β in lithium-induced nephropathic rats S Saberi, MA Rajizadeh, M Khaksari, A Saber,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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