Rat Interleukin 12,IL-12/P40 ELISA KIT

The rat IL-12 ELISA kit is suitable for the quantitative determination of rat IL-12 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

IL-12 is a Th1 cytokine predominantly produced by cells in the innate immune system. Its role is linked to the differentiation of naïve T cells into Th1 cells. In allergy, IL-12 is known to downregulate allergic inflammation following its release, along with IFNγ. It modulates the expression of many genes involved in effector T cell function such as FasL and granzyme B. IL-12 signals through the IL-12R activate a STAT4-dependent intracellular pathway, leading to the production of IFNγ but also directly resulting in IL-18 synthesis. It is important for cancer therapy to provide a crucial bridge between innate and adaptive immunity.