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Rabbit β-Thromboglobulin,β-TG ELISA Kit

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Code: CSB-E09285Rb
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
Pls inquire
Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 12.5 ng/mL-800 ng/mL
Sensitivity: 3.12 ng/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
beta-Thromboglobulin, beta-TG
Abbreviation
β-TG
Species
Oryctolagus cuniculus (Rabbit)
Sample Type
serum, plasma, tissue homogenates
Detection Range
12.5 ng/mL-800 ng/mL
Sensitivity
3.12 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rabbit β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
800 2.801 2.869 2.835 2.748
400 2.484 2.398 2.441 2.354
200 1.716 1.768 1.742 1.655
100 0.944 0.968 0.956 0.869
50 0.454 0.468 0.461 0.374
25 0.256 0.248 0.252 0.165
12.5 0.147 0.151 0.149 0.062
0 0.085 0.089 0.087 　
Troubleshooting and FAQs
ELISA kit FAQs
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Description

This Rabbit β-TG ELISA Kit was designed for the quantitative measurement of Rabbit β-TG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 5 reviews

Submit a Review here

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:6:"Rabbit";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E09285Rb to detect the content of β-TG in normal rabbit serum, and the R2 of the standard curve could be as high as 0.9921. The standard curve met the requirements, and the OD values of the samples were 0.264 and 0.268, which could be parallel, and the test results were accurate and perfect.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:6:"Rabbit";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E09285Rb to detect the content of β-TG in the serum of normal rabbits. The R2 of the standard curve could be greater than 0.99, and the OD value of the sample was in line with the range, and the detection result was accurate and perfect.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:6:"Rabbit";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E09285Rb to detect the content of β-TG in normal rabbit serum, and the R2 of the standard curve could be as high as 0.9921. The standard curve met the requirements, and the OD values of the samples were 0.264 and 0.268, which could be parallel, and the test results were accurate and perfect.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:3:"Rat";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E09285Rb to detect normal plasma. The 0D value of the standard curve was about 3.x-0.06x, and the OD value of the sample was about 0.2-0.1. The standard curve R of the kit was >0.99, which met the test requirements and the kit was very good.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:6:"Rabbit";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E09285Rb to detect normal plasma, the standard curve was more than 2 points, S0 was about 0.06, the OD value of the sample was about 0.3, the standard curve r of the kit was >0.99, which met our requirements and was well received.

By Anonymous

■ Q&A
Q:

I would like to redirect another rabbit assay to your assay because it is based on antibodies against the human sequence and not rabbit.
I understand that immunogen information for your kits is not normally revealed however if we are going to redirect another assay to your kit we need to know that the antibodies in your kit are directed against the rabbit sequence. If not and they are against a different species then please reveal that species and how cross reactivity has been confirmed.

A:
Thanks for your inquiry.
About Rabbit beta-Thromboglobulin, pls refer to:
https://www.ncbi.nlm.nih.gov/protein/XP_008266105.1
For this kit CSB-E09285Rb, the standard is 293 cell-derived recombinant protein.
Pls let me know if you have any further questions. Thank you.

Citations

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