Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

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Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

Name: Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit
Brand: CUSABIO
SKU: CSB-E14220Rb

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Code: CSB-E14220Rb

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 93.75 pg/mL-6000 pg/mL

Sensitivity: 23.44 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

MMP13Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit

Abbreviation

MMP13

Uniprot NO.

O62806

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma, tissue homogenates

Detection Range

93.75 pg/mL-6000 pg/mL

Sensitivity

23.44 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	105
	Range %	97-108
1:4	Average %	90
	Range %	85-95
1:8	Average %	95
	Range %	89-100

Recovery

The recovery of rabbit MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	98	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.554	2.444	2.499	2.405
3000	1.937	1.976	1.957	1.863
1500	1.409	1.422	1.416	1.322
750	0.815	0.789	0.802	0.708
375	0.422	0.434	0.428	0.334
187.5	0.302	0.289	0.296	0.202
93.75	0.207	0.198	0.203	0.109
0	0.093	0.095	0.094

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Rabbit MMP13 ELISA Kit was designed for the quantitative measurement of Rabbit MMP13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.44 pg/mL.

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Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone Li Y. et al,/,2015
Chondroprotective and antiarthritic effects of daphnetin used in vitro and in vivo osteoarthritis models Xiaohan Zhang, et al,/,2019
In Vitro and In vivo approaches to evaluate Uncaria tomentosa bark extract loaded FDOFs on Osteoarthritis models JN Sowjanya - 2023 - researchsquare.com,/,2023

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

Gene References into Functions

These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. PMID:27697532
the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID:23879595
chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID:23520014
Leptin can induce MMP-13 and have a synergistic induction effect on NO with TNF-alpha in rabbit articular chondrocytes in vitro. PMID:19893248