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Rabbit Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

Rabbit Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

CSB-E04625Rb

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 7.8 pg/mL-500 pg/mL

Sensitivity: 1.95 pg/mL

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Orders can only be placed from within the U.S.

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Product Details

Alternative Names

MIP-1 alpha,Macrophage Inflammatory Protein-1 alpha

Abbreviation

MIP1α/CCL3

Species

Oryctolagus cuniculus (Rabbit)

Sample Type

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	93-109
1:2	Average %	110
	Range %	97-114
1:4	Average %	88
	Range %	83-93
1:8	Average %	95
	Range %	85-98

Recovery

The recovery of rabbit MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	95-109
EDTA plasma (n=4)	101	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.803	2.736	2.770	2.629
250	2.362	2.455	2.409	2.268
125	1.743	1.862	1.803	1.662
62.5	1.123	1.151	1.137	0.996
31.25	0.730	0.744	0.737	0.596
15.6	0.550	0.537	0.544	0.403
7.8	0.306	0.312	0.309	0.168
0	0.139	0.143	0.141