Pig collagen type I (COLI) ELISA kit

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Code: CSB-EQ027853PI
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 78 pg/mL-5000 pg/mL
Sensitivity: 19.5 pg/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
collagen type 1 (COL1) ,collagen type I (COLI)
Abbreviation
COLI
Species
Sus scrofa (Pig)
Sample Type
serum, plasma, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig COLⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 88    
Range % 86-91    
1:2 Average % 89    
Range % 81-95    
1:4 Average % 98    
Range % 93-104    
1:8 Average % 102    
Range % 98-106    
Recovery
The recovery of pig COLⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 104 94-109    
EDTA plasma (n=4) 92 88-98    
               
               
Typical Data
Pig collagen type I (COLI) ELISA kit
Troubleshooting and FAQs
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  • Pig collagen type I (COLI) ELISA kit
Description

This Pig COLI ELISA Kit was designed for the quantitative measurement of Pig COLI protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Customer Reviews and Q&A

 Customer Reviews
 Q&A
Q:

I would like to confirm that the COL1 ELISA Kit, is specific for pig collagen type 1

A:
Thanks for your inquiry!The kit csb-eq027853pi specifically detects pig collagen type I.

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