Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

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Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

Name: Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit
Brand: CUSABIO
SKU: CSB-E08676m

CSB-E08676m

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma

Detect Range: 0.625 ng/mL-40 ng/mL

Sensitivity: 0.156 ng/mL

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Orders can only be placed from within the U.S.

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Abbreviation

MAC Ab

Species

Mus musculus (Mouse)

Sample Type

serum, plasma

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody （MPO-ANCA）in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	95
	Range %	83-101
1:4	Average %	96
	Range %	90-106
1:8	Average %	94
	Range %	82-101

Recovery

The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody（MPO-ANCA）spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.


Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-98
EDTA plasma (n=4)	94	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.404	2.418	2.411	2.297
20	1.956	1.904	1.930	1.816
10	1.352	1.377	1.365	1.251
5	0.902	0.934	0.918	0.804
2.5	0.526	0.534	0.530	0.416
1.25	0.311	0.326	0.319	0.205
0.625	0.265	0.271	0.268	0.154
0	0.112	0.115	0.114

Troubleshooting and FAQs

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Datasheet & COA

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Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit

Description

This Mouse MAC Ab ELISA Kit was designed for the quantitative measurement of Mouse MAC Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.