Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

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Name: Mouse glucagon-like peptide-1,GLP-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08118m

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Code: CSB-E08118m

Rate （4.8）
Review （5）
Citation （16）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates.

Detect Range: 125 pg/ml-8000 pg/ml.

Sensitivity: 31.25 pg/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Product Details
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Target Background

Alternative Names

N/A

Abbreviation

GLP-1

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates.

Detection Range

125 pg/ml-8000 pg/ml.

Sensitivity

31.25 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	98-110
1:2	Average %	95
	Range %	91-105
1:4	Average %	101
	Range %	94-108
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

8000

2.397

2.334

2.365

2.173

4000

1.701

1.667

1.684

1.492

2000

1.162

1.098

1.130

0.938

1000

0.788

0.819

0.803

0.611

500

0.572

0.551

0.562

0.370

250

0.413

0.423

0.418

0.226

125

0.302

0.294

0.298

0.106

0.189

0.195

0.192

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The Mouse glucagon-like peptide-1 (GLP-1) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of mouse GLP-1in many biological fluids, including serum, plasma, or tissue homogenates. GLP-1 is a gut-derived peptide secreted from intestinal L-cells following a meal. The action of GLP-1 is through the binding of a specific GLP-1 receptor, which is expressed in various tissues, including pancreatic beta cells, pancreatic ducts, and gastric mucosa. GLP-1 exerts its main effect by enhancing glucose-dependent insulin production. GLP-1 has also been demonstrated to delay gastric emptying, suppress inappropriate post-meal glucagon release, and increase satiety.

This assay employs the Sandwich-ELISA technique, in which GLP-1 in the samples or standards is sandwiched between pre-coated an anti-mouse GLP-1 and Biotin-labeled GLP-1 antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of GLP-1 in the sample. The kit has a detection range of 62.5 ng/mL-4000 ng/mL, excellent sensitivity, high specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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