Mouse anti-double stranded DNA antibody (IgG) ELISA Kit

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Code: CSB-E11194m
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum
Detect Range:
Sensitivity:
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Abbreviation
dsDNA Ab (IgG)
Species
Mus musculus (Mouse)
Sample Type
serum
Detection Range
Sensitivity
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
qualitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Indirect
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Typical Data
 
Troubleshooting and FAQs
Description

The mouse anti-double-stranded DNA antibody (IgG) ELISA kit can quantitatively detect anti-double-stranded DNA (anti-dsDNA) antibodies. It operates through an indirect measurement method. The kit allows for the detection of anti-dsDNA antibodies in mouse serum samples, with an assay time ranging from 1 to 5 hours and a sample volume requirement of 50-100 µl. The detection wavelength is at 450 nm. This kit has been utilized in more than 13 papers, underlining its significance and reliability in research settings. Studies have shown that the presence of self-DNA drives the production of IgG anti-dsDNA antibodies in lupus, highlighting the importance of tools like this ELISA kit in understanding autoimmune mechanisms. Anti-dsDNA antibodies are highly specific for systemic lupus erythematosus (SLE). These antibodies are crucial in predicting disease exacerbation in SLE.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 3 reviews

Submit a Review here

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E11194m to detect the expression of IgG in the plasma of normal and SLE model mice. The OD values after 1:10 dilution ranged from 0.19 to 0.649, and the measured results were at low concentrations. The product is easy to use, with detailed instructions. It is recommended to perform pre-tests for different samples to find out the concentration range before performing large batch experiments.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:4:"1:10";}}

Review: I tested dsDNA in mice with the CSB-E11194m kit.

By Anonymous

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:4:"1:20";}}

Review: I use CSB-11194m to detect autoantibodies in mouse plasma, the results of this elisa kit are very stable, and all the people around me use Huamei\'s kit. I have used other kits before, but I feel that they are not as reliable as Huamei\'s. I will continue to use them in the future, which is very convenient for our research. We will continue to use it in the future, which is very convenient for our research.

By Anonymous

 Q&A
Q:

1. What is the standard in the kit?
If this is a recombinant protein, what is the expression method?
Is it expressed in E.coli?
2. What is the stop solution? We need to know the material and quantity.

A:
Thanks for your inquiry.
The standard of this kit CSB-E11194m is the native sample crude extract(recombinant protein)
Stop solution: 4N sulfuric acid
The detailed kit components and quantity, pls check the matarials provided part on page 4 from the manual.I will send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.

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