Mouse Interferon γ ,IFN-γ ELISA Kit

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Target Background

Name: Mouse Interferon γ ,IFN-γ ELISA Kit
Brand: CUSABIO
SKU: CSB-E04578m-IS

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Code: CSB-E04578m-IS

Rate （5.0）
Review （1）
Citation （1）

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates, cell culture supernates.

Detect Range: 1.56 pg/ml-100 pg/ml

Sensitivity: 0.39 pg/ml

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Alternative Names

Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit

Abbreviation

IFNG

Uniprot NO.

P01580

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates, cell culture supernates.

Detection Range

1.56 pg/ml-100 pg/ml

Sensitivity

0.39 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-98
1:2	Average %	97
	Range %	90-104
1:4	Average %	91
	Range %	85-96
1:8	Average %	101
	Range %	94-108

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

100

3.000

3.134

3.067

2.880

2.297

2.178

2.238

2.051

1.259

1.348

1.303

1.116

12.5

0.838

0.829

0.834

0.647

6.25

0.537

0.561

0.549

0.362

3.12

0.367

0.353

0.360

0.173

1.56

0.285

0.274

0.280

0.093

0.189

0.185

0.187

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This kit provides quantitative measurement of interferon-gamma (IFN-γ) in mouse samples, supporting immunological research into Th1-mediated responses, infection, and inflammatory disease. Because IFN-γ is often produced transiently and at low concentrations—particularly in unstimulated or early-phase immune responses—this kit is designed to capture biologically meaningful differences across diverse experimental conditions.

Detection Range: The 1.56–100 pg/ml range aligns with physiological IFN-γ levels in mouse serum and stimulated splenocyte supernatants, where concentrations typically fall in the low-to-mid pg/ml range. Samples from strongly activated T cells or NK cells (e.g., anti-CD3/CD28 stimulation, LCMV infection models) may require dilution to remain within range.

Sensitivity: At 0.39 pg/ml, the kit detects IFN-γ in low-abundance contexts such as naïve mouse serum, early post-infection time points, or conditioned media from small numbers of sorted Th1 or CD8⁺ T cells.

Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants enables paired in vivo/ex vivo study designs—for example, correlating splenic homogenate levels with circulating IFN-γ or restimulation assay readouts.

Assay Efficiency: The 1–5 hour protocol and 50–100 µl sample volume accommodate experiments constrained by limited primary cell yields or small-volume bleeds from longitudinal mouse studies.

This kit is directly applicable to studies of intracellular infection clearance, graft rejection, and Th1/Th2 polarization.

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:4:"1:10";}}

Review: I used CSB-E04578m-IS to detect interferon-γ in the serum of mice. I bought the ornate kit for the first time and followed the instructions step by step to operate. Finally, the results did not get the desired statistical difference, but there was a corresponding trend, and we still need to do further verification. Tell me some feelings about this kit. 1. The description is particularly detailed and can be made step by step. It is relatively simple, but the reagent is written in English name. Before you use it, you must determine which is which, don\'t worry about using it wrong. 2. Biotin-labeled antibody and horseradish peroxidase labeled avidin are relatively few, so we should pay special attention when adding samples, especially accurate! It is better to vortex the vortex attached to the wall at the beginning. When I added it, I made 48 samples, and I think I used 2/3 of the two samples. Next time, I will have to contact the customer service here to see if I can add 3. The standard curve in the final result is very good, and the R square is greater than 0.99, but there are some large differences within my group, which may be related to the washing plate operation. I\'ll have to try again recently, but the trend in my six groups as a whole is definitely visible. Some information on the official website is really good, the standard curve processing software is also very professional, but it is possible to input x one by one to get the y value, this is relatively large. You must watch the video and instructions before you do it, don\'t just do it!! Touch the dilution concentration and do a pre-experiment at the same time. I hope I can get good results next! Also hope you can have good experimental results!! Come on, researchers!

By Anonymous

Surface Display of Type 1 Fimbriae on Shigella flexneri Induces Antigen-Specific Immune Response via Oral Route S Sang, R Yu, Y Mao, Y Zhai, C Cao, K Li, Y Guan,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.

Gene References into Functions

Data show that interleukin-2 inducible T cell kinase (Itk)negatively regulates the development of nTh1 cells that express interferon-gamma (IFNgamma) in a T-bet transcription factor (Tbet) independent manner. PMID:28406139
demonstrate in vivo that the conditional overexpression of Ifng in metanephric mesenchymal (MM) progenitors results in renal agenesis or hypoplasia. Cell death was observed in and around the MM region of E10.5-11.5 mutants where Ifng was constitutively expressed during early kidney development and resulted in retardation of branching morphogenesis. Expression of Sall1 was decreased in the MM of mutant kidneys. PMID:29771971
Data confirm Ifng as robust supporter of immune responses against tumors; here, mice treated with Chlorella vulgaris probiotic supposed as anti-carcinogen against mammary tumor, instead tumors in treated group exhibit more malignant phenotype and lower peri-tumoral neutrophil and macrophage-to-lymphocyte infiltration ratio compared to control mice; decline in serum Ifng levels correlated with tumor growth. PMID:28229276
In interferon gamma (IFNgamma)-deficient mice, Akkermansia muciniphila is significantly increased and restoration of IFNgamma levels reduces A. muciniphila abundance. PMID:27841267
this study shows the positive effects of Ifng on the early-stage differentiation and negative effects on the calcification of primary osteoblasts in vitro PMID:29438885
these data support the novel concept that IFN-gamma can have a detrimental role in the pathogenesis of influenza through a restriction in innate lymphoid cell group II activity PMID:28513592
IFN-gamma-iNOS axis are an essential pathway in the pathogenesis of arenavirus hemorrhagic fever. PMID:28826838
IFN-gamma can promote cancer immunoevasion. (Review) PMID:29283429
IRF-1 may be at the nexus of the interplay between IFN-gamma and IL-6 in exacerbating a xenobiotic-induced inflammatory response, regulation of interferon responsive genes and autoimmunity PMID:28453771
an enhanced expression of PD-L1 was observed besides an increased production of IFN-gamma by TH2 cells. PMID:28917991
Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID:29133416
SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID:29180417
The complex role of IFN-gamma in autoimmunity and cholangitis.IFN-gamma induces IL-30 production which suppresses IFN-gamma mediated liver inflammation. PMID:27721424
this study shows that IFN-gamma promotes transendothelial migration of CD4(+) T cells across the blood-brain barrier PMID:28682305
Addition of a disintegrin and metallopeptidase domain 17 (ADAM17) to the culture supernatant of stimulated splenocytes decreased Interferon-gamma (IFN-gamma) concentration. PMID:27573075
a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID:28849104
the protective effect on metastasis was lost upon patrolling monocyte or NK cell depletion, IL15 neutralization, or IFNgamma ablation. The combined analysis of these approaches allowed us to establish a hierarchy in which patrolling monocytes, making IL15 in response to primary tumors, activate NK cells and IFNg production that then inhibit lung metastasis formation. PMID:28811289
Interferon-gamma derived from cytotoxic lymphocytes directly enhances their motility and cytotoxicity. PMID:28569770
this paper shows that interferon-gamma deficiency protects against aging-related goblet cell loss PMID:27623073
IL-15 induces the activation and survival of effector immune cells that are necessary for its antitumoral activity; but, long-term exposure to IL-15 is associated with the development of important side effects mainly mediated by IFN-gamma-producing T-cells PMID:27356750
Arid5a deficiency resulted in decreased levels of IFN-gamma under Th1 cell conditions, in which T-box expressed in T cells (T-bet) mRNA expression was inhibited. PMID:27671645
High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID:28092793
these findings highlight critical roles for IFN-gamma and IL-27 in the mechanism of respiratory syncytial virus-induced exacerbation PMID:29167232
MyD88 signaling in myeloid and dendritic cells is dispensable for IFN-gamma-dependent control of type A F. tularensis infection. PMID:28951422
spleen-derived IFN-gamma induces generation of PD-L1(+)-suppressive neutrophils. PMID:28974543
These results suggest that IL-17A plays an important role in host survival against Toxoplasma gondii infection by protecting the host from an anaphylactic reaction via the downregulation of Toxoplasma gondii HSP70 and IFN-gamma production. PMID:28893913
Stat3 mediates the expression of iNOS to promote IFNgamma/TNFalpha-induced muscle atrophy. PMID:28264935
NFAT5 can modulate different T-cell responses depending on stress conditions and stimulatory context. PMID:27479742
we identify interferon-gamma (IFN-gamma) as the key inflammatory mediator controlling sortilin-1 levels PMID:28742217
this study shows that IFN-gamma induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense PMID:26349661
IFNG mediates experimental cerebral malaria by signaling within both the hematopoietic and nonhematopoietic compartments. PMID:28874445
identified TIM-4 as a novel marker for B effector 1 (Be1) cells that depend on IFN-gamma for their proinflammatory activity; TIM-4(+) B cells are enriched for IFN-gamma-producing proinflammatory Be1 cells that enhance immune responsiveness and can be specifically targeted with anti-TIM-4. PMID:28848066
results demonstrate that a population of Thy1.2(+) non-NK innate-like cells present in the liver expresses IFN-gamma and can confer protection against M. avium infection in immunocompromised mice PMID:28687660
Using IFN-gamma-deficient Th17 cells, study demonstrates the disease-amplifying role of Th17-derived IFN-gamma in dry eye disease pathogenesis. These results clearly demonstrate that Th17 cells mediate ocular surface autoimmunity through both IL-17A and IFN-gamma. PMID:28637904
IFNgamma is captured by phosphatidylserine (PS) on the surface of viable tumor cells both in vitro and in vivo then slowly released to drive long-term transcription of cytokine-response genes. PMID:28575659
Egr2 and 3 were essential to suppress Th1 differentiation in Th2 and Th17 conditions in vitro and also to control IFN-gamma-producing CD4 and CD8 T cells in response to virus infection PMID:28455436
intravital microscopy revealed IFNgamma-induced regression of the tumour vasculature, resulting in arrest of blood flow and subsequent collapse of tumours, similar to non-haemorrhagic necrosis in ischaemia PMID:28445461
studies identify the requirement of IFN-gamma stimulation as a mechanism for BC-CML and AML GVL resistance, whereas independence from IFN-gamma renders CP-CML more GVL sensitive, even with a lower-level alloimmune response. PMID:28604385
Results demonstrate that the influence of IFN-gamma on acute lymphoblastic leukemia progression may not be mediated by selection of nascent transformed cells but rather through a general suppressor of cytokine signaling -mediated reduction in B-cell precursor proliferation. PMID:28295300
The results indicate that interferon-gamma is critical for the initial priming and differentiation of cytotoxic T lymphocytes residing in the periphery to produce the most effect antitumor function within the eye. PMID:26578649
The authors showed the synergistic negative regulation of the pro-inflammatory cytokine interferon-gamma (IFNgamma) and beta3 integrin signaling in murine hematopoietic stem cells function by a novel definitive phenotyping of hematopoietic stem cells. PMID:28673932
We revealed that in mouse mesenchymal stem cells IFN-gamma-induced immunoregulation is mediated by early phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3, which is significantly enhanced by an extracellular signal-regulated kinase 1/2-dependent mTOR inhibition, thereby promoting pSTAT1 nuclear translocation. PMID:27670240
this study showsthat the nature of the protective memory response depends closely on the route of infection and highlights the role of IFN-gamma-and IL-17RA-mediated responses in the control of mucosal infection by Brucella PMID:27036913
Study showed that the intraperitoneal administration of the exogenous cytokines IFN-gamma (to promote M1 microglia ) and IL-4 (to promote M2 microglia) can correctly modulate the timing of the M1 to M2 ratio to affect epileptogenesis and to improve cognitive function in pilocarpine-induced status epilepticus. PMID:27956120
findings show that bystander activation of T cells through TLRs leads to a short but biologically significant burst of IFN-gamma production, which relies exclusively on newly synthetized transcripts and energy generated by mitochondrial respiration PMID:27016606
Study finds that a high proportion of intratumoral Nrp1-/- Tregs produce interferon-gamma (IFNgamma), which drives the fragility of surrounding wild-type Tregs, boosts anti-tumor immunity, and facilitates tumor clearance. PMID:28552348
results demonstrate that Th1 cell-driven responses in the intestine during chronic helminth infection potently influence upstream hematopoietic processes in the Bone Marrow via IFN-gamma. PMID:27594558
Data show that lactic acid in tumor microenvironments inhibited interferon-gamma (IFNgamma) and intert=leukin-4 (IL4) productions from NKT cells by inhibiting mammalian target of rapamycin (mTOR) signaling. PMID:27995420
These findings reveal an unexpected tolerogenic role for IFN-gamma during viral persistence in the liver, providing new mechanistic insights regarding the maintenance of systemic antigen-specific tolerance during HBV persistence. PMID:27139489
CD4+CD25+ regulatory T cells promoted IFN-gamma and IL-17 mycoplasma-specific CD4+ T cell responses in vitro and in vivo. PMID:27175511