Mouse Free Thyroxine,FT4 ELISA Kit

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Code: CSB-E05080m
Size:
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 4 pmol/L-64 pmol/L
Sensitivity: 1.08 pmol/L
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Abbreviation
FT4
Species
Mus musculus (Mouse)
Sample Type
serum, plasma, tissue homogenates
Detection Range
4 pmol/L-64 pmol/L
Sensitivity
1.08 pmol/L
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Competitive
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision

Intra-assay Precision (Precision within an assay): CV%<15%

 

Three samples of known concentration were tested twenty times on one plate to assess.

 

Inter-assay Precision (Precision between assays): CV%<15%

 

Three samples of known concentration were tested in twenty assays to assess.

  

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting and FAQs
Images
  • Mouse Free Thyroxine,FT4 ELISA Kit
  • Mouse Free Thyroxine,FT4 ELISA Kit
Description

Free thyroxine (FT4) represents the unbound, metabolically active fraction of thyroxine hormone in circulation, constituting approximately 0.03% of total thyroid hormone. As the primary hormone produced by the thyroid gland, FT4 regulates cellular metabolism, growth, and development throughout the body. Unlike total thyroxine measurements, FT4 levels provide a more accurate picture of thyroid function since they remain unaffected by variations in thyroid-binding proteins. This makes FT4 an important biomarker in endocrine research and thyroid disorder studies.

The Mouse Free Thyroxine FT4 ELISA Kit (CSB-E05080m) measures FT4 concentrations in mouse serum, plasma, and tissue homogenates. This competitive assay operates within a detection range of 4-64 pmol/L with a sensitivity of 1.08 pmol/L. The kit requires 50-100 μL sample volume and provides results within 1-5 hours using spectrophotometric detection at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining thyroid hormone regulation and endocrine function in mouse models. Studies have applied the kit to measure free thyroxine levels in serum samples as part of broader hormonal profiling approaches. The kit supports research examining metabolic and endocrine pathways where thyroid hormone status serves as a key parameter.

Endocrine profiling - Used alongside other hormone measurements to determine thyroid function status in experimental studies
Metabolic research - Applied in studies examining relationships between thyroid hormones and metabolic parameters including lipid profiles
Stress response studies - Used to evaluate thyroid hormone levels in conjunction with stress-related hormones in serum samples
Comprehensive hormone panels - Integrated into multi-analyte approaches measuring various endocrine markers simultaneously

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:5:"Serum";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:11:"No dilution";i:1;s:0:"";}}

Review: I used CSB-E05080m to detect the serum FT4 value of normal mice, and the OD value was 1.7016 1.9263 1.8087, which was convenient to use and the instructions were clear.

By Anonymous

 Q&A
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

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