Mouse Apolipoprotein B48 (apo-B48) ELISA Kit

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Code: CSB-E16506m
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 62.5 ng/mL-4000 ng/mL
Sensitivity: 15.6 ng/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Abbreviation
apo-B48
Species
Mus musculus (Mouse)
Sample Type
serum, plasma, tissue homogenates
Detection Range
62.5 ng/mL-4000 ng/mL
Sensitivity
15.6 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Competitive
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-B48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 96  
Range % 92-104  
1:400 Average % 93  
Range % 89-97  
1:800 Average % 87  
Range % 84-92  
1:1600 Average % 87  
Range % 82-93  
Recovery
The recovery of mouse apo-B48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-99  
EDTA plasma (n=4) 98 94-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
4000 0.073 0.074 0.074    
2000 0.117 0.120 0.119    
1000 0.183 0.192 0.188    
500 0.283 0.275 0.279    
250 0.451 0.448 0.450    
125 0.770 0.768 0.769    
62.5 1.048 1.122 1.085    
0 2.275 2.371 2.323    
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  • Mouse Apolipoprotein B48 (apo-B48) ELISA Kit
Description

This Mouse apo-B48 ELISA Kit was designed for the quantitative measurement of Mouse apo-B48 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.

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