Human glucagon-like peptide-1,GLP-1 ELISA Kit

CUSABIO's GLP-1 human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLP-1 in serum, plasma, or tissue homogenates. This assay uses an antibody specific to human GLP-1 coated on a 96-well plate. GLP-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated GLP-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of GLP-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

GLP-1 is a gastrointestinal peptide that is produced by the enteroendocrine L cells and is released in response to meal intake. GLP-1 mainly functions to stimulate insulin secretion and to suppress food intake, gastric emptying, and glucagon secretion, thereby limiting postprandial glucose excursions. It is important for glucose homeostasis. It also inhibits gastrointestinal motility and secretion and thus acts as an enterogastrone and part of the "ileal brake" mechanism. GLP-1 stimulates the proliferation, differentiation, and neogenesis of β cells.