Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

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Code: CSB-E09606h
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma
Detect Range: 0.312 ng/mL-20 ng/mL
Sensitivity: 0.078 ng/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Target Name
cytokeratin fragment antigen 21-1,CYFRA21-1
Abbreviation
CYFRA21-1
Species
Homo sapiens (Human)
Sample Type
serum, plasma
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-90  
1:2 Average % 98  
Range % 91-105  
1:4 Average % 99  
Range % 94-110  
1:8 Average % 92  
Range % 86-99  
Recovery
The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 80-98  
EDTA plasma (n=4) 97 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.541 2.385 2.463 2.305  
10 2.229 2.082 2.156 1.998  
5 1.607 1.545 1.576 1.418  
2.5 0.866 0.842 0.854 0.696  
1.25 0.463 0.439 0.451 0.293  
0.625 0.337 0.324 0.331 0.173  
0.312 0.254 0.246 0.250 0.092  
0 0.160 0.156 0.158    
Troubleshooting and FAQs
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  • Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit
Description

This Human CYFRA21-1 ELISA Kit was designed for the quantitative measurement of Human CYFRA21-1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

Customer Reviews and Q&A

 Customer Reviews
 Q&A
Q:

May I know are the antibodies used in the ELISA kit polyclonal antibodies or monoclonal antibodies?
Human cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit

A:
Thanks for your inquiry.Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.

Target Background

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