Human advanced glycation end products,AGEs ELISA Kit

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Human advanced glycation end products,AGEs ELISA Kit

Name: Human advanced glycation end products,AGEs ELISA Kit
Brand: CUSABIO
SKU: CSB-E09412h

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Code: CSB-E09412h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.78 μg/mL-50 μg/mL

Sensitivity: 0.195 μg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Abbreviation

AGEs

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.78 μg/mL-50 μg/mL

Sensitivity

0.195 μg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AGEs in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	90
	Range %	85-95
1:4	Average %	94
	Range %	90-99
1:8	Average %	104
	Range %	100-109

Recovery

The recovery of human AGEs spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
50	2.698	2.652	2.675	2.534
25	2.076	2.082	2.079	1.938
12.5	1.204	1.283	1.244	1.103
6.25	0.639	0.669	0.654	0.513
3.12	0.388	0.374	0.381	0.240
1.56	0.261	0.259	0.260	0.119
0.78	0.187	0.179	0.183	0.042
0	0.145	0.137	0.141

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Human advanced glycation end products,AGEs ELISA Kit

Description

The human advanced glycation end products (AGEs) ELISA kit is designed to quantitatively measure AGEs in human samples. This ELISA kit is specific for the human species and is suitable for analyzing serum, plasma, and tissue homogenates. The minimal detection level of this kit is 0.195 μg/mL. The AGEs detection range of the kit is 0.78 μg/mL to 50 μg/mL. It operates on a sandwich method, where AGEs are captured between two antibodies. Typically, the assay takes 1 to 5 hours, with a sample volume requirement of 50-100 μg. The detection is performed at a wavelength of 450 nm. More than 19 papers have used this kit to support their experimental data.