Human activated protein C,APC ELISA Kit

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Code: CSB-E09909h
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 1.56 pg/mL-100 pg/mL
Sensitivity: 0.39 pg/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
N/A
Abbreviation
APC
Species
Homo sapiens (Human)
Sample Type
serum, plasma, tissue homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
0.39 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 87-96  
1:200 Average % 94  
Range % 90-97  
1:400 Average % 97  
Range % 94-103  
1:800 Average % 94  
Range % 90-98  
Recovery
The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-94  
EDTA plasma (n=4) 87 84-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.755 2.990 2.873 2.754  
50 2.065 2.201 2.133 2.014  
25 1.359 1.288 1.324 1.205  
12.5 0.853 0.816 0.835 0.716  
6.25 0.470 0.473 0.472 0.353  
3.12 0.325 0.302 0.314 0.195  
1.56 0.184 0.192 0.188 0.069  
0 0.115 0.122 0.119    
Troubleshooting and FAQs
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  • Human activated protein C,APC ELISA Kit
Description

This Human APC ELISA Kit was designed for the quantitative measurement of Human APC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

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