SARS-CoV-2 Neutralizing Antibody ELISA Kit

Neutralizing antibodies (NAbs) are a type of special antibodies with the capacity of neutralizing viral toxicity. The invasion of SARS-CoV-2 into host cells depends on the interaction between the viral S protein and the ACE2 receptor on host cells. NAbs against SARS-CoV-2 specifically bind to the RBD within the S protein of SARS-CoV-2, blocking RBD-ACE2 receptor interaction, thus preventing the virus infect the host cells and clearing the virus. For more details about Neutralizing antibodies, please read this article: https://www.cusabio.com/c-21014.html

1. Prepare all reagents, samples, and working standards as instructed.
2. Refer to the Assay Layout Sheet to calculate the number of the wells (including a blank well) to be used and put the remaining wells and the desiccant back into the pouch to seal and store at 4 °C.
3. Add 50 µl of standard or sample (may require dilution) to each well, followed by 50 µl of HRP-conjugated ACE2 (not to the blank well). Shake the plate gently for 1 minute. Seal and incubate for 1 hour at  37 °C.
4. After thorough washing, add 90 μl of TMB Substrate Solution to each well. Incubate for 20 minutes at 37°C.
5. Add 50 µl Stop Solution to each well and gently tap the plate to ensure thorough mixing.
6. Read the OD (optical density) value at 450 nm within 5 minutes using a microplate reader.