SARS-CoV-2 Neutralizing Antibody ELISA Kit

CSB-EL33243HU

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96T,5×96T,10×96T

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Sample Types: serum, plasma

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Product Details

Alternative Names

2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2

Abbreviation

SARS-CoV-2 neutralizing Ab

Species

Homo sapiens (Human)

Sample Type

serum, plasma

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Infectious Diseases

Assay Principle

qualitative

Measurement

Competitive

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10000	0.099	0.092	0.096
5000	0.145	0.136	0.141
2500	0.194	0.183	0.189
1250	0.274	0.263	0.269
625	0.344	0.371	0.358
312	0.519	0.528	0.524
156	0.901	0.907	0.904
78	1.509	1.412	1.461
39	1.787	1.723	1.755
19.5	2.121	1.958	2.040
9.75	2.233	2.138	2.186
0	2.394	2.328	2.361

Troubleshooting and FAQs

ELISA kit FAQs

Datasheet & COA

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Description

CUSABIO's SARS-CoV-2 Neutralizing Antibody ELISA Kit is intended for qualitative measurement of neutralizing antibodies in human serum or plasma. SARS-CoV-2 neutralizing antibodies directly target the SARS-CoV-2 S receptor-binding domain (RBD), blocking the protein's interaction with the host ACE2 receptor, thus interfering with the viral attachment to the cell as well as its entry into the cell. When using the reference neutralizing antibody provided in the kit, the assay has a detection range of 9.75-10000 ng/ml, a sensitivity of as low as 9.75 ng/ml, and Intra- /Inter-assay Precision low than 15%. This detection kit is simple in operation and scalable in sample size (high throughput). Multiple validations display it has excellent sensitivity and high specificity. It is conducive to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection. And it should not be used for the diagnosis of acute SARS-CoV-2 infection.

Customer Reviews and Q&A

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How Do SARS-CoV-2 Neutralizing Antibodies Work?

Neutralizing antibodies (NAbs) are a type of special antibodies with the capacity of neutralizing viral toxicity. The invasion of SARS-CoV-2 into host cells depends on the interaction between the viral S protein and the ACE2 receptor on host cells. NAbs against SARS-CoV-2 specifically bind to the RBD within the S protein of SARS-CoV-2, blocking RBD-ACE2 receptor interaction, thus preventing the virus infect the host cells and clearing the virus. For more details about Neutralizing antibodies, please read this article: https://www.cusabio.com/c-21014.html

Why should I choose the SARS-CoV-2 neutralizing antibody ELISA kit from CUSABIO?

1. Simple, convenient and fast;
2. Operation can be completed in any laboratory setting;
3. Strong specificity, small inter-assay variation.

What is the principle of the SARS-CoV-2 Neutralizing Antibody ELISA Kit?

This assay is based on competitive method. The kit detects circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain (RBD) of the viral spike glycoprotein with the ACE2 cell surface receptor.

Which applications can I use the SARS-CoV-2 neutralizing antibody ELISA kit for?

1. Viral spike glycoprotein (RBD)/ACE2 receptor interaction studies;
2. Vaccine and Biologics development;
3. COVID-19 Epidemiology Studies.

What is the assay procedure?

1. Prepare all reagents, samples, and working standards as instructed.
2. Refer to the Assay Layout Sheet to calculate the number of the wells (including a blank well) to be used and put the remaining wells and the desiccant back into the pouch to seal and store at 4 °C.
3. Add 50 µl of standard or sample (may require dilution) to each well, followed by 50 µl of HRP-conjugated ACE2 (not to the blank well). Shake the plate gently for 1 minute. Seal and incubate for 1 hour at 37 °C.
4. After thorough washing, add 90 μl of TMB Substrate Solution to each well. Incubate for 20 minutes at 37°C.
5. Add 50 µl Stop Solution to each well and gently tap the plate to ensure thorough mixing.
6. Read the OD (optical density) value at 450 nm within 5 minutes using a microplate reader.