Human Interleukin 12,IL-12/P70 ELISA Kit

Product Details

Name: Human Interleukin 12,IL-12/P70 ELISA Kit
Brand: CUSABIO
SKU: CSB-E04599h-IS

CSB-E04599h-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates

Detect Range: 3.9 pg/ml-250 pg/ml

Sensitivity: 0.975 pg/ml

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Target Background

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Abbreviation

IL-12/P70

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell culture supernates

Detection Range

3.9 pg/ml-250 pg/ml

Sensitivity

0.975 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	97-111
1:2	Average %	92
	Range %	85-99
1:4	Average %	96
	Range %	90-101
1:8	Average %	90
	Range %	83-97

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

250

2.333

2.434

2.383

2.196

125

1.229

1.297

1.263

1.075

62.5

0.853

0.828

0.840

0.652

31.25

0.571

0.559

0.565

0.377

15.6

0.345

0.358

0.351

0.164

7.8

0.287

0.273

0.280

0.092

3.9

0.240

0.238

0.239

0.051

0.191

0.185

0.188

Troubleshooting and FAQs

ELISA kit FAQs

Datasheet & COA

Please contact us to get it.

Description

This Human IL-12/P70 ELISA Kit was designed for the quantitative measurement of Human IL-12/P70 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.9 pg/ml-250 pg/ml and the sensitivity is 0.975 pg/ml.