Human Estradiol,E2 ELISA Kit

Product Details

Name: Human Estradiol,E2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E05108h

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Code: CSB-E05108h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma.

Detect Range: 50 pg/ml-1200 pg/ml.

Sensitivity: 40 pg/ml.

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Target Background

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Alternative Names

N/A

Abbreviation

Species

Homo sapiens (Human)

Sample Type

serum, plasma.

Detection Range

50 pg/ml-1200 pg/ml.

Sensitivity

40 pg/ml.

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

1.952

1.834

1.893

1.493

1.567

1.530

120

1.045

1.028

1.037

250

0.679

0.629

0.654

500

0.419

0.421

0.420

1200

0.234

0.243

0.239

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

The human Estradiol (E2) ELISA kit is a powerful tool for quantitative measurement of estradiol levels in human serum and plasma samples, with a detection range of 50 pg/ml to 1200 pg/ml and a sensitivity of 40 pg/ml. The assay principle is competitive, meaning that two antibodies competitively bind to the estradiol in the sample. The detection is read at a wavelength of 450 nm. The assay time typically ranges from 1 to 5 hours, requiring a sample volume of 50-100 µl. The kit's performance has been validated in multiple studies found in more than 18 publications, ensuring its accuracy and reproducibility in measuring estradiol levels in human samples.