Human Epinephrine/Adrenaline,EPI ELISA Kit

Product Details

Name: Human Epinephrine/Adrenaline,EPI ELISA Kit
Brand: CUSABIO
SKU: CSB-E08677h

Promotion

Code: CSB-E08677h

Rate （N/A）
Review （0）
Citation （9）

Compare

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 2 pg/mL-400 pg/mL

Sensitivity: 1 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Customer Reviews and Q&A
Target Background

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Abbreviation

EPI

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

2 pg/mL-400 pg/mL

Sensitivity

1 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-96
1:8	Average %	90
	Range %	84-98

Recovery

The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	80-94
EDTA plasma (n=4)	98	92-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
400	0.213	0.210	0.212
100	0.408	0.391	0.400
32	0.613	0.631	0.622
8	1.062	1.003	1.033
2	1.593	1.516	1.555
0	2.467	2.412	2.440

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Images

Human Epinephrine/Adrenaline,EPI ELISA Kit

Description

The human Epinephrine/Adrenaline (EPI) ELISA kit provides a means for quantitatively measuring Epinephrine levels in human serum, plasma, and tissue homogenates. With a detection range of 2 pg/mL to 400 pg/mL and a sensitivity of 1 pg/mL, this kit allows for precise and accurate measurements of Epinephrine levels. This content looks too robotic. The assay principle is competitive, and the detection wavelength is set at 450 nm, ensuring reliable and reproducible results. The assay time ranges from 1 to 5 hours, making it efficient for laboratory use, and requires a sample volume of 50-100 µl, which is suitable for conserving precious samples. The utility of this ELISA kit is further supported by its application in several studies in multiple articles. The kit's ability to accurately measure Epinephrine levels in various biological samples makes it a valuable tool for research in fields such as stress, diabetes, and cardiovascular diseases.