Human Calcitonin,CT ELISA Kit

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Name: Human Calcitonin,CT ELISA Kit
Brand: CUSABIO
SKU: CSB-E05131h

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Code: CSB-E05131h

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 4.688 pg/mL-300 pg/mL

Sensitivity: 1.172 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

CALCA ELISA kit; CALC1Calcitonin [Cleaved into: Calcitonin; Katacalcin ELISA kit; Calcitonin carboxyl-terminal peptide ELISA kit; CCP ELISA kit; PDN-21)] ELISA kit

Abbreviation

CALCA

Uniprot NO.

P01258

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

4.688 pg/mL-300 pg/mL

Sensitivity

1.172 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	92-100
1:4	Average %	94
	Range %	90-110
1:8	Average %	94
	Range %	89-105

Recovery

The recovery of human CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-100
EDTA plasma (n=4)	92	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.379	2.252	2.316	2.173
150	1.794	1.760	1.777	1.634
75	1.305	1.284	1.295	1.152
37.5	0.954	0.909	0.932	0.789
18.7	0.593	0.564	0.579	0.436
9.4	0.372	0.349	0.361	0.218
4.7	0.253	0.239	0.246	0.103
0	0.149	0.137	0.143

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

■ Customer Reviews

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Blood biomarkers for occupational hand-arm vibration exposure P Vihlborg,/,2022
Validity of Anti-nuclear Antibodies (ANA), double strand DNA (dsDNA) and other parameters in Autoimmune hyperparathyroidism THH Al-Tai¹, HH Al-Ammiri, AA Mahdi,/,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.; Katacalcin is a potent plasma calcium-lowering peptide.

Subcellular Location

Secreted.

Protein Families

Calcitonin family

Database Links

HGNC: 1437

UNIGENE: Hs.37058

KEGG: hsa:796

STRING: 9606.ENSP00000331746

OMIM: 114130

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