Fish adrenocorticotropic hormone (ACTH) ELISA kit

Product Details

Name: Fish adrenocorticotropic hormone (ACTH) ELISA kit
Brand: CUSABIO
SKU: CSB-E15926Fh

CSB-E15926Fh

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 75 pg/mL-1200 pg/mL

Sensitivity: 75 pg/mL

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Orders can only be placed from within the U.S.

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Target Background

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Abbreviation

ACTH

Species

Fish

Sample Type

serum, plasma, tissue homogenates

Detection Range

75 pg/mL-1200 pg/mL

Sensitivity

75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	94-109
1:2	Average %	92
	Range %	84-97
1:4	Average %	101
	Range %	98-105
1:8	Average %	95
	Range %	84-98

Recovery

The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	82-97
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1200	0.149	0.152	0.151
600	0.300	0.288	0.294
300	0.477	0.491	0.484
150	0.871	0.889	0.880
75	1.835	1.887	1.861

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Datasheet & COA

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Fish adrenocorticotropic hormone (ACTH) ELISA kit

Description

This Fish ACTH ELISA Kit was designed for the quantitative measurement of Fish ACTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 75 pg/mL-1200 pg/mL and the sensitivity is 75 pg/mL.