Canine Testosterone (T) ELISA Kit

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Code: CSB-E06893c
Size:
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 0.1 ng/mL-20 ng/mL
Sensitivity: 0.05 ng/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
N/A
Abbreviation
T
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Type
serum, plasma, tissue homogenates
Detection Range
0.1 ng/mL-20 ng/mL
Sensitivity
0.05 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Competitive
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %91
Range %86-97
1:2Average %97
Range %92-103
1:4Average %85
Range %81-89
1:8Average %89
Range %83-95
Recovery
The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-95
EDTA plasma (n=4)8782-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
200.232 0.236 0.234
50.435 0.436 0.436
1.60.611 0.613 0.612
0.40.984 0.978 0.981
0.11.401 1.403 1.402
01.985 1.989 1.987
Troubleshooting and FAQs
Description

Testosterone is a steroid hormone that plays important roles in reproductive physiology, sexual development, and secondary sexual characteristics across mammalian species. As the primary male sex hormone, testosterone regulates spermatogenesis, libido, and the development of male reproductive tissues, while also influencing muscle mass, bone density, and behavioral patterns. In veterinary and comparative endocrinology research, testosterone measurements are necessary for studying reproductive health, breeding programs, and hormonal disorders in domestic and wild animal populations.

The Canine Testosterone ELISA Kit (CSB-E06893c) provides quantitative measurement of testosterone in canine samples using a competitive assay principle. This kit works with serum, plasma, and tissue homogenates with a detection range of 0.1-20 ng/mL and sensitivity of 0.05 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Measurements are taken at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This canine testosterone ELISA kit has been used in veterinary endocrine research to quantify testosterone levels in various biological samples. The kit supports hormonal profiling studies and androgen-related research in canine subjects. Applications span reproductive health research and prostatic disease studies where testosterone measurement is needed for understanding hormonal status.

Hormonal profiling - Used alongside estrogen measurements to assess reproductive hormone levels in canine research studies
Prostatic disease research - Applied in conjunction with dihydrotestosterone and estradiol analysis to study hormonal factors in prostate-related conditions
Androgen studies - Used for testosterone quantification in serum and tissue samples as part of comprehensive hormonal assessments
Veterinary endocrine research - Applied in laboratory studies examining hormonal biomarkers alongside other prostatic-specific markers

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