SARS-CoV-2 Spike RBD Antibody Pair 1

Code: CSB-EAP33245

Rate （5.0）
Review （2）
Citation （2）

Size:

1 pair

US$1000

Quantity:

Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: S-ELISA

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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P0DTC2

Alternative Names

S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Llama with human IgG1 Fc

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Protein Names

Human Novel Coronavirus Spike glycoprotein (S)

Host

Capture: Llama with human IgG1 Fc
Detection: Mouse with human IgG1 Fc

Buffer

Capture:50% Glycerol, 0.01M PBS, PH 7.4
Detection: 50% Glycerol, 0.01M PBS, PH 7.4

Components

Capture: CSB-EAP33245C
Detection: CSB-EAP33245B(HRP)
Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.

Tested Applications

S-ELISA

Notes

We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml.Optimal dilutions should be determined experimentally by the researcher.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

CSB-EAP33245 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Spike RBD has been pre-coated onto the microwells. The SARS-CoV-2 Spike RBD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Spike RBD is added to detect the captured SARS-CoV-2 Spike RBD protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

Submit a Review here

Applications : Enzyme-linked immunosorbent assay (ELISA)

Sample type: Vero cells

Review: Optimization of analytical conditions. (A) Optimization of spike protein antigen concentration from 1 µg mL−1 to 20 µg mL−1, (B) Effect of binding time between spike protein antigen and Ni(OH)2 NPs from 15 min to 65 min, (C) Effect of binding time between antibody and immobilized antigen from 5 min to 30 min, (each measurement was performed 3 times and the RSD averaged 1.5%).

By Anonymous

Applications : Optimization of analytical experiments

Sample type: Human

By Anonymous

■ Q&A

Have both antibodies been raised against the RBD domain of the S1 protein?

Yes, both capture antibody and detection antibody are specific for RBD of S1 protein.

Which protein would you recommend as the standard for an ELISA assay?

We recommend the Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial (CSB-MP3324GMY) as standards for an ELISA assay.

Are there any recommendations for the concentrations of the different points of the standard curve?

Our experimental data of standards concentration as well as OD values is as below.

I am looking for a pair of antibodies for SARS-COV2 detection by a sandwich assay. Do you have any available?

Cusabio supplies the following matched antibody pair for detecting SARS-CoV-2 Spike protein in Sandwich ELISA assay. Cusabio guarantees that the antibody works for the applications/species stated on our website and datasheet, a replacement or refund will be provided if the antibody does not work as described on our datasheet.

CSB-EAP33245 SARS-CoV-2 Spike RBD Antibody Pair 1 https://www.cusabio.com/Antibody-Pairs/S-Antibody-Pair-12928623.html
Concentration:
Capture Antibody: 1mg/ml
Detection Antibody: 0.42mg/ml

If you also need a standard protein, we would like to recommend the following product.
CSB-MP3324GMY1b1 https://www.cusabio.com/Recombinant-Protein/Recombinant-Human-Novel-Coronavirus-Spike-glycoprotein-S---partial--12928577.html

Citations

Target Background

Function(From Uniprot)

attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.; May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.

Gene References into Functions

Study presents crystal structure of C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike S protein in complex with human ACE2 (hACE2); hACE2-binding mode similar overall to that observed for SARS-CoV. However, details at the binding interface show that key residue substitutions in SARS-CoV-2-CTD slightly strengthen the interaction and lead to higher affinity for receptor binding than SARS-CoV receptor-binding domain. PMID:32378705
crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2 PMID:32365751
crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2 PMID:32320687
Out of the two isolates from India compared to the isolates from Wuhan, China, one was found to harbor a mutation in its receptor-binding domain (RBD) at position 407 where, arginine was replaced by isoleucine. This mutation has been seen to change the secondary structure of the protein at that region and this can potentially alter receptor binding of the virus. PMID:32275855
Structural modeling of the SARS-CoV-2 spike glycoprotein show similar receptor utilization between SARS-CoV-2 and SARS-CoV, despite a relatively low amino acid similarity in the receptor binding module. Compared to SARS-CoV and all other coronaviruses in Betacoronavirus lineage B, an extended structural loop containing basic amino acids were identified at the interface of the receptor binding (S1) and fusion (S2) domains. PMID:32245784
crystal structure of CR3022, a neutralizing antibody from a SARS patient, in complex with the receptor-binding domain of the SARS-CoV-2 spike (S) protein to 3.1 A; study provides insight into how SARS-CoV-2 can be targeted by the humoral immune response and revealed a conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV PMID:32225176
SARS-CoV and SARS-CoV-2 spike proteins have comparable binding affinities achieved by balancing energetics and dynamics. The SARS-CoV-2-ACE2 complex contains a higher number of contacts, a larger interface area, and decreased interface residue fluctuations relative to the SARS-CoV-ACE2 complex. PMID:32225175
Interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. Authors identified that N82 of ACE2 showed closer contact with receptor-binding domain of S protein than human ACE2. PMID:32221306
SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs; determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer. PMID:32201080
Study demonstrates that SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. PMID:32155444
The ACE2-B0AT1 complex exists as a dimer of heterodimers. Structural alignment of the RBD-ACE2-B0AT1 ternary complex with the S protein of SARS-CoV-2 suggests that two S protein trimers can simultaneously bind to an ACE2 homodimer. PMID:32142651
study demonstrated SARS-CoV-2 S protein entry on 293/hACE2 cells is mainly mediated through endocytosis, and PIKfyve, TPC2 and cathepsin L are critical for virus entry; found that SARS-CoV-2 S protein could trigger syncytia in 293/hACE2 cells independent of exogenous protease; there was limited cross-neutralization activity between convalescent sera from SARS and COVID-19 patients PMID:32132184
study determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation; provided biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S PMID:32075877

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Subcellular Location

Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.

Protein Families

Betacoronaviruses spike protein family

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